heatmap generated using graphpad prism software  (GraphPad Software Inc)

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Development of new adeno-associated virus capsid variants for targeted gene delivery to human cardiomyocytes

doi: 10.1016/j.omtm.2023.08.010

Figure Lengend Snippet: Cardiotropic rAAV variants obtained by directed evolution were closely related to WT AAV6 and AAV3 Directed evolution was performed using the hiPSC-CM line WTCWT. After six rounds of selection, the enriched novel AAVs were sequenced and phylogenetic analysis performed to compare with parental AAVs. (A) Clustering of amino acid sequences from novel variants (red) to WT AAV parental serotypes (black), with relationships depicted as a phylogenetic tree. (B) Heatmap illustrating the percentage identity among select parental AAVs and enriched novel variants. (C) Parental sequence contribution analysis of variants isolated after directed evolution. Black line represents the most probable composition of individual shuffled clones based on the longest sequence of identity to parental variants in a 5′ to 3′ direction. (D) Surface representations of the new capsid variants KK01, KK02, KK03, and KK05. The colored regions (yellow, orange, purple) denote surface residues that differ from the parental AAV6 capsid. Amino acid changes not located on the capsid surface are listed below. (D) Depiction as in (E) for the capsid variant KK04. The colored regions (red and purple) denote surface residues that differ from the parental AAV3b capsid. See also Figure S1 .

Article Snippet: Amino acid substitutions were also displayed using a heatmap generated from GraphPad Prism v.8.2.1 for Windows (GraphPad Software, Boston, MA).

Techniques: Selection, Sequencing, Isolation, Clone Assay, Variant Assay